IVF Treatment gives Parental Life

About ICSI (Intra Cytoplasmic Sperm Injection)

IVF (test tube baby) has been as established medical practice since 1978, when the first IVF baby was born in the United Kingdom. But men with low sperm count or absent sperm count could not get help from this treatment because, for IVF a healthy sperm count with good sperm motility and morphology is needed to achieve successful outcome. So awaiting the early 90s, men with very low sperm count or poor quality sperm had no hope of fathering their own children and had to resort to donor sperm. This problem was surmounted by the new breakthrough of intra cytoplasmic sperm injection (ICSI), which took place in Brussels and Belgium in 1992. Since then, millions of babies worldwide have been born with the help of this technique.

Process for ICSI:

In ICSI all the steps are similar to the procedure of IVF, except fertilization. Normally in IVF, one egg is mixed with approximately 200,000 sperms and one of the sperm fertilizes the egg on its own. In ICSI each egg is held and injected with a single live sperm. This micro fertilization is done with the help of a machine called the micro-manipulator.

The procedure involves controlled ovarian stimulation in the female partner with hormonal medications to produce multiple follicles which are then monitored for egg development by pelvic sonography and serial estradiol hormone estimation. The next step is administration of HCG injection (Human Chorionic Gonadotropin) when at-least two leading follicles are 18mm in diameter. Oocyte or egg retrieval is done under short general anesthesia, after 35 to 36 hours of HCG injection. The next step involves identification and isolation of eggs in the IVF laboratory followed by collection of sperm and processing it in the lab. Hyaluronidase enzyme helps in splitting the cumulus of the eggs which are then placed in small droplets of culture media under oil.

Next a micro-injection needle is used for absorption of the immobile sperm into the needle after crushing the sperm tail portion followed by injection of the immobilized sperm into the held egg, which are placed into the incubator for two to five days. After embryo formation two to five days after fertilization the good quality embryos are transferred back to the womb.

Men with low sperm count, very poor motility or high degree of abnormal sperms and even those having a lack of sperm in the semen can undergo this technique. ICSI can also be performed in males with sperm anti bodies, ejaculation dysfunction due to spinal cord injury or malfunction such as quadriplegics or paraplegics and also those suffering from retrograde ejaculation (ejaculation of the sperm into the urinary bladder) and in cases where multiple in vitro fertilization has failed.

For more details please contact us through our Email: info@kiranivfgenetic.com