ICSI Treatment in Hyderabad :

Until the 90’s, males with very low sperm count (less than 5 million per ml) or with poor quality sperms had
no hope of fathering their own genetic children. This problem was circumvented by a new breakthrough,
I.C.S.I(Intra-cytoplasm Sperm Injection) an ART Treatment which took place in Brussels and Belgium in 1992.

Since then, many such patients with poor sperm count or with low quality sperm have been able to father their own genetic child through ICSI treatment in Hyderabad.At Kiran infertility centre, we started our own ICSI programmed in 1998-99 and until date, more than 10,000 cycles of IVF or IUI with ICSI procedure in Hyderabadhave been undertaken with an approximate success rate of around 60% to 80% in each cycle, which is at par with the best success percentages in this treatment across the globe.

In ICSI, all the steps are similar to that in the IVF procedure, except the step of fertilization. Normally in IVF, extracted sperms, which are around, or more than 200,000 and allowed to fuse with one egg and among these, one of the sperm fertilizes the egg on its own. In contrast to this, while undertaking ICSI, each egg is held and injected with a single live sperm. This micro-fertilization is done with the help of a machine called the Micromanipulator. The procedure can be categorized into 11 steps as discussed below.

icsi treatment in hyderabad

Thus, the procedure consists of:

ICSI Treatment in India

  • Controlled ovarian stimulation with drugs like (GnRH) Analogues and Gonadotrophins to produce multiple follicles/ eggs
  • Monitoring of follicles and egg development with the aids like Vaginal Sonography and serial Estradiol hormone estimation through blood
  • Administering of HCG injection, (Human Chorionic Gonadotrophins) when at-least two leading follicles, which are 18 mm indiameter
  • Egg retrieval under general anaesthesia, approximately after 36 hours after HCGinjection
  • Identification and isolation of healthy eggs in thelaboratory
  • Sperm collection and processing in the lab (In case of Azoospermia {no sperms in the semen} the sperms are collected directly from the testes with the procedures of PESA/MESA/TESE or TESA)
  • Stripping of the cumulus of the eggs in the laboratory with the help of an enzyme Placement of eggs into small droplets of culture media underoil
  • Placing sperms into small droplets of PVP under oil and then Immobilization of the sperm with a microinjection needle (Diameter of 7 microns) and aspiration of the immobile sperm into the needle after crushing the tail portion of thesperm)
  • Holding the egg with a holding pipette and injecting of immobilized sperm into the heldegg
  • Placement of these eggs into the incubator for 2 to 5days
  • Embryo formation 2 to 5 days afterfertilization
  • Embryo transfer of good quality embryos back to the womb, on day-2 (four-cell embryo), day-3 (6 to 8 cell embryo) or day-5 or 6 (Blastocyst stage) days after egg

Indications for ICSI

  • Males with severe sperm factors such as low sperm count (less than 5 million), very poor motility or a high degree of abnormal sperms require Intra Cytoplasmic Sperm Injection treatment in
  • In Azoospermia Condition, where there is no sperm present in the semen. Azoospermia may be  of the obstructive type where there is a production of sperms in the testes but blockage of the transport system, which brings the sperm out into the semen. Alternately, in Azoospermia condition may be of the non-obstructive type, where there is a failure of the testes to produce sperms. In current scenario, in both these conditions, sperms can be isolated directly from the testis by Testicular
  • Retrieval Techniques of PESA/TESA/TESE and subsequently, ICSI can be performed on the followingcases:
    • Males with Sperm Ejaculated Dysfunction due to Spinal injury or malfunction such as Quadriplegics orParaplegics
    • Males with Antisperm-antibodies
    • Patients with Retrograde Ejaculation (ejaculation of the sperm into the urinary bladder) who fail to become pregnant with
    • Patients where fertilization has failed with In-VitroFertilization
ICSI treatment in hyderabad

At Kiran infertility centre, we also suggest Intra cytoplasmic sperm injection/icsi on patients who have had a previous history of Tuberculosis or Endometriosis, as with our experience, we are of a view that it has an opportunity of better fertilization rates than standard IVF procedure.

ICSI Treatment Cost in Hyderabad

Intra cytoplasmic sperm injection has revolutionised the way Fertility treatments were conducted in the past. ICSI treatment in Hyderabad is world class and offered at the Kiran infertility centre in Hyderabad/bangalore and Chennai wherein even the most difficult of male infertility cases can be treated successfully.